The Efficacy of Different Laser Applications on Dentin Sealing in Preventing Discoloration Induced by Mineral Trioxide Aggregate.

The aim of this in vitro study was to evaluate the effect of the pre-application of a dentin-bonding agent and the application of different lasers on the prevention of tooth discoloration caused by mineral trioxide aggregate (MTA) in the presence of blood. Sixty extracted human anterior teeth were selected, with root lengths standardized to 10 mm and root canals shaped using Gates-Glidden drills #3 to #5. The samples were divided into six groups (n = 10): Group 1 with no surface treatment and Groups 2 to 6 with Optibond universal adhesive and Neodymium yttrium aluminum garnet (Nd:YAG), Erbium yttrium aluminum garnet (Er:YAG), Erbium-chromium-yttrium-scandium-gallium-garnet (Er:Cr:YSGG), and diode laser applications, respectively. Root canals were filled with fresh human blood, and ProRoot MTA and a collagen barrier were then placed. Color changes were measured with a spectrophotometer at 0, 7, 30, 90, and 180 days post MTA placement. Color differences (∆E) were analyzed using a two-factor mixed-design ANOVA with the Sidak method (p = 0.05). All treatment groups exhibited discoloration above the acceptability threshold. Although all treatment approaches exhibited less color change compared to the control group (p < 0.05), there was no significant difference among them in terms of preventing color change (p > 0.05). It was determined that none of the methods could guarantee 100% prevention of discoloration caused by MTA-blood contact.

Efficacy of different irrigant activation devices in removing dentin debris from an artificial isthmus connecting curved canals.

The aim of this study was to evaluate the debridement efficacy of sonic irrigation (EDDY; VDW, Munich, Germany and EndoActivator; Dentsply-Sirona, Ballaigues, Switzerland) and ultrasonically activated irrigation (Irrisafe; Satelec Acteon, Merignac, France) in a simulated canal isthmus connecting curved canals. Transparent resin blocks were produced containing two curved canals connected with an isthmus. The isthmus was then filled with dentin debris. Three irrigant activation cycles were performed and the amount of remaining debris was compared analysing pictures taken after each activation cycle. Data were statistically analysed using one-way ANOVA and post hoc Tukey tests at a significance level of p < 0.05. EDDY showed greater efficiency in removing dentin debris from the simulated isthmus than the other techniques tested. An increase in the activation time enhanced the efficiency of both EDDY and ultrasonically activated irrigation in debris removal.

Investigation of the effectiveness of sonic, ultrasonic and new laser-assisted irrigation activation methods on smear removal and tubular penetration.

The aim of this study was to examine the effectiveness of different irrigation activation methods on smear layer removal and tubular penetration. One hundred-five distal roots of mandibular molar teeth in total; 50 for smear removal efficiency (n = 10) analysis using scanning electron microscopy (SEM), and 55 roots were used to examine tubular penetration using confocal laser scanning microscope (CLSM). Five different irrigation activation methods were used in this study; conventional needle irrigation (CNI), sonic irrigation device of EDDY, passive ultrasonic irrigation (PUI), PIPS and SWEEPS techniques, which are two different laser irrigation activation methods. The obtained data were statistically analyzed and the significance level was determined as p < 0.05. At the apical level, the cleanest canal walls were observed when laser methods PIPS and SWEEPS were used, while in the middle third, there was no difference in smear removal efficiencies between all groups except for the CNI (p > 0.05). Penetration depths and percentages increased from apically to coronally in all groups. The PUI and EDDY generally showed similar penetration depths and percentages to the CNI, except at the coronal root level (p > 0.05). In all groups, when PIPS was used, it showed greater penetration depth and percentage (p < 0.05). PIPS and SWEEPS techniques showed lowest and similar smear scores compared to PUI and EDDY in the apical area where access and effectiveness of the irrigation solution are difficult.

Investigation of the effect of indirect pulp capping materials on dentin mineral density

Aim: To evaluate the potential of inducing mineral density changes of indirect pulp capping materials applied to demineralized dentin. Methods: A total of 50 cavities were prepared, 5 in each tooth, in extracted ten molars without caries, impacted or semi-embedded. The cavities were scanned by microcomputed tomography (µ-CT) after creating artificial caries by microcosm method (pre-treatment). Each cavity was subjected to one of 5 different experimental conditions: control (dental wax), conventional glass ionomer cement (Fuji IX GP Extra), resin-modified calcium silicate (TheraCal LC), resin-modified calcium hydroxide (Ultra-Blend Plus), MTA (MM-MTA) and the samples were kept under intrapulpal pressure using simulated body fluid for 45 days. Then, the second µ-CT scan was performed (post-treatment), and the change in dentin mineral density was calculated. Afterward, elemental mapping was performed on the dentinal surfaces adjacent to the pulp capping agents of 5 randomly selected samples using energy dispersive X-ray spectroscopy (EDS) apparatus attached to a scanning electron microscope (SEM). The Ca/P ratio by weight was calculated. Friedman test and Wilcoxon Signed Ranks test were used to analyze the data. Results: There was a significant increase in mineral density values of demineralized dentin after treatment for all material groups (p<0.05). Resin-modified calcium silicate had similar efficacy to MTA and conventional glass ionomer cement, but was superior to resin-modified calcium hydroxide in increasing the mineral density values of demineralized dentin. Conclusions: Demineralized dentin tissue that is still repairable can be effectively preserved using materials with remineralization capability

Mechanical Properties of the New Generation RACE EVO and R-Motion Nickel-Titanium Instruments.

This study aimed to evaluate and compare the dynamic cyclic fatigue, torsional and bending resistance of two novel RACE EVO (FKG Dentaire SA, La Chaux de Fonds, Switzerland) and R-Motion (FKG) nickel−titanium instruments with traditional RaCe (FKG) instruments. RACE EVO, R-Motion and RaCe instruments with a size of 25 and taper of 0.06 were used. A dynamic cyclic fatigue test was used to assess the time to fracture. The fractured surfaces were further analyzed using scanning electron microscopy at ×350 and ×3000 magnifications. A torsional resistance test was performed to measure the maximum torsional strength and angle of rotation. Phase transformations with temperature were evaluated using differential scanning calorimetry. The results were statistically analyzed with a Kruskal−Wallis test at a 5% significance level. R-Motion had the highest time to fracture and the lowest torsional and bending resistance, whereas RaCe had the lowest time to fracture and the highest torsional and bending resistance (p < 0.05). In relation to the angle of rotation, RACE EVO instruments had the highest deformation capacity followed by R-Motion and RaCe instruments (p < 0.05). The greater cyclic fatigue resistance and lower torsional and bending resistance results indicate that the novel R-Motion and RACE EVO instruments are less rigid and more flexible than RaCe instruments.

Postoperative pain after SWEEPS, PIPS, sonic and ultrasonic-assisted irrigation activation techniques: a randomized clinical trial.

To investigate the efficacy of a new laser irrigation activation system [shock wave-enhanced emission photo-acoustic streaming (SWEEPS)] in terms of postoperative pain after primary root canal treatment compared with other techniques, namely photon-induced photo-acoustic streaming (PIPS), sonic system with EDDY, passive ultrasonic system (PUI), and manual dynamic activation (MDA). Two hundred patients with symptomatic irreversible pulpitis in mandibular premolar teeth were enrolled and randomly allocated to five different irrigation activation groups (n = 40) after chemo-mechanical root canal preparation. For irrigation activation, the SWEEPS and PIPS tips were attached to the Er-YAG laser system in the respective groups, while the Irrisafe tip was used in the PUI group and the EDDY tip in the sonic group. In the MDA group, irrigation was agitated with the master gutta-percha cone. At postoperative hours 8, 24, and 48 and on day 7, pain intensity was evaluated using the 10-mm Visual Analog Scale (VAS) and analgesic intake was recorded. The Kruskal-Wallis test was used to analyze the VAS scores, and pain prevalence and analgesic intake were examined with the Pearson's chi-square test at the 5% significance level. The PIPS and SWEEPS groups had the lowest level and prevalence of pain when compared to the remaining groups (P < 0.001). The PUI, sonic and MDA groups did not differ in terms of pain scores at hours 8 and 48 (P > 0.05). On day 7, the highest score and pain prevalence were recorded in the MDA group (P < 0.001). There was no difference between the groups in terms of analgesic intake (P > 0.05). Laser-activated irrigation systems provided lower postoperative pain scores and levels compared to the other activation systems. The MDA group had the highest pain scores and incidence at the end of the seventh day.

Postoperative pain after different irrigation activation techniques: a randomized, clinical trial.

The aim of this study was to assess the effectiveness of irrigation activation techniques on postoperative pain (PP) in mandibular premolar teeth with irreversible pulpitis after single-visit endodontic treatment. A total of 160 patients with symptomatic irreversible pulpitis were included in this prospective randomized clinical study. Four different activation methods were used in mandibular premolar teeth. In group 1, teeth were irrigated with side-port endodontic needles (NI) without any agitation; in groups 2 and 3, sonic activation was performed using EDDY and EndoActivator (EA), respectively; and in group 4, passive ultrasonic irrigation (PUI) was used. Patients' analgesic intake-as well as pain intensity during and after treatment-were recorded at 8, 24, 48 h and 7 days. The data relating to age, sex and analgesic intake was evaluated using the Chi-square test and the preoperative pain and PP intensity at different time intervals was evaluated with the Kruskal-Wallis test at a 5% significance level. Highest PP was recorded at 8 h, pain intensity decreased in all groups by the time. Pain in the NI group was found higher than that of EDDY group at 24 h (P < 0.05). EA and PUI had caused mild pain and had similar pain scores at 24 h. (P > 0.05). No statistically difference was found among the groups with regard to analgesic intake (P > 0.05). Although there were slight differences in PP levels between the groups at 24 h, pain levels decreased in all groups after 24 h. Activation of the irrigation solution did not make any difference in terms of PP after 24 h.

Cyclic fatigue resistance of reduced-taper nickel-titanium (NiTi) instruments in doubled-curved (S-shaped) canals at body temperature.

Background. This study was conducted to compare the cyclic fatigue resistance of VDW.ROTATE, TruNatomy Prime, HyFlex CM, and 2Shape nickel-titanium (NiTi) rotary instruments in double-curved canals in a simulated clinical environment. Methods. Eighty NiTi files were used for the fatigue testing in stainless steel canals compatible with instrument sizes until fracture occurred (n=20): VDW.ROTATE (04./25#), TruNatomy Prime (04./26#), HyFlex CM (04./25#) and 2Shape TS04./25#( 1). For each instrument, the number of cycles to fracture (NCF) was calculated, and the fractured fragment length (FL) was measured. To verify that the files were fractured due to cyclic fatigue, the fractured surfaces of the files were evaluated under a scanning electron microscope. Data were statistically analyzed using the Kruskal-Wallis and Student's t-tests at the %95 confidence level. Results. The failure of the files due to cyclic fatigue was first seen in the apical curvature before the coronal curvature (P<0.05). The highest fatigue resistance was observed in VDW.ROTATE and HyFlex CM files in both curvatures (P<0.05). There were no significant differences in the fatigue resistance between the HyFlex CM and VDW.ROTATE files or between the 2Shape and the TruNatomy files (P>0.05). There was no difference in the fractured lengths of the files between the apical and coronal curvatures (P>0.05). Conclusion. In artificial S-shaped root canals, VDW.ROTATE and HyFlex CM files exhibited higher fatigue resistance compared to 2Shape and TruNatomy files.

Comparison of the cyclic fatigue resistance of VDW.ROTATE, TruNatomy, 2Shape, and HyFlex CM nickel-titanium rotary files at body temperature.

OBJECTIVES: This study aims to compare the cyclic fatigue resistance of VDW.ROTATE, TruNatomy, 2Shape, and HyFlex CM nickel-titanium (NiTi) rotary files at body temperature. MATERIALS AND METHODS: In total, 80 VDW.ROTATE (25/0.04), TruNatomy (26/0.04), 2Shape (25/0.04), and HyFlex CM (25/0.04) NiTi rotary files (n = 20 in each group) were subjected to static cyclic fatigue testing at body temperature (37°C) in stainless-steel artificial canals prepared according to the size and taper of the instruments until fracture occurred. The number of cycles to fracture (NCF) was calculated, and the lengths of the fractured fragments were measured. The data were statistically analyzed using a 1-way analysis of variance and post hoc Tamhane tests at the 5% significance level (p < 0.05). RESULTS: There were significant differences in the cyclic fatigue resistance among the groups (p < 0.05), with the highest to lowest NCF values of the files as follows: VDW.ROTATE, HyFlex CM, 2Shape, and TruNatomy. There was no significant difference in the lengths of the fractured fragments among the groups. The scanning electron microscope images of the files revealed typical characteristics of fracture due to cyclic fatigue. CONCLUSIONS: The VDW.ROTATE files had the highest cyclic fatigue resistance, and the TruNatomy and 2Shape files had the lowest cyclic fatigue resistance in artificial canals at body temperature.

Fracture strength of endodontically treated teeth restored with different fiber post and core systems.

The aim of this study was to investigate the effects of different post and core systems, CAD/CAM crown placement on fracture strength of endodontically treated mandibular premolar teeth. One hundred forty single-rooted premolar teeth were randomly divided into a control group and six experimental groups as follows: control group (Group 1); FiberSite post luting with Clearfil DC Core Plus (Group 2); RelyX Fiber post luting and core build up with Clearfil DC Core Plus (Group 3); RelyX Fiber post luting with Clearfil DC Core Plus and core build up with Filtek Bulk Fill Posterior (Group 4); specimens that received CAD/CAM crowns after the same procedures performed in Groups 2, 3, and 4, respectively (Groups 5, 6, and 7). Fracture strength tests were performed, and the failure modes were recorded. Data were statistically analyzed using one-way ANOVA and post hoc Tukey tests. The highest fracture resistance was observed in the control group and among the experimental groups in Group 4. Groups 3 and 5 showed similar fracture resistance followed by Group 2. The lowest fracture resistances were seen in the samples of Groups 6 and 7. While the fracture strength of Group 2 increased after the crown placement (Group 5), the fracture strength of Group 3 and Group 4 decreased (P < 0.05). While the specimens in Groups 1, 2, 3, and 4 predominantly showed favorable failure, unfavorable failure was more frequent in Groups 5, 6, and 7.

Surface deformation of several nickel-titanium rotary instruments after removal of the root canal filling materials: a scanning electron microscope evaluation.

The aim of the present study was to investigate and compare the surface deformations of different NiTi rotary files after retreatment under scanning electron microscope (SEM). A total of 240 upper central incisor teeth were obturated with vertical compaction technique and divided into 6 groups. Root canals were retreated by ProTaper Next (PTN), Twisted File Adaptive (TFA), Reciproc (RPC), ProTaper Universal Retreatment (PTR), R-Endo and Mtwo-R NiTi rotary systems. The files investigated under SEM at different magnifications and photomicrographs were captured. The photomicrographs were classified and scored. Kruskal-Wallis and Dunn tests were performed for statistical analyze at 5% significance level. PTR and RPC groups were free of defects like file separation, curving and unwinding but PTN and TFA groups showed 50% file separation. According to the photomicrographs that were captured at high magnification; there were no statistically difference between groups at the coronal thirds of the files (P > 0.05), at the middle third PTN and TFA groups showed statistically more deformation than the other groups (P < 0.05). At the apical third Reciproc was statistically showed less deformation than the other all groups (P < 0.05). Within the limitation of this in vitro study, all NiTi files showed surface deformation in SEM observation after retreatment procedures. There was less deformation in PTR and RPC groups according to other.

Cyclic fatigue resistance of HyFlex EDM, Reciproc Blue, WaveOne Gold, and Twisted File Adaptive rotary files under different temperatures and ambient conditions.

Background. This study examined the effects of changes in temperature and environmental conditions on the cyclic fatigue resistance of Reciproc Blue, HyFlex EDM, WaveOne Gold, and Twisted File Adaptive. Methods. Forty-five Reciproc Blue (25/.08), 45 HyFlex EDM (25/.08), 45 WaveOne Gold (25/.07), and 45 Twisted File Adaptive (25/.08) files were tested for cyclic fatigue at room temperature (20°C) in air and water and at body temperature (35°C) in water. All the instruments were rotated in artificial canals made of stainless steel with an inner diameter of 1.5 mm, 60° angle of curvature and a radius of curvature of 5 mm until fracture occurred; the time to fracture was recorded in seconds, using a digital chronometer. Mann-Whitney U test was used for the statistical analysis of data, with SPSS 21.0. Results. Cyclic fatigue resistance was significantly higher in all the groups in water at an ambient temperature of 20°C compared with air and water at temperatures of 20°C and 35°C, respectively (P<0.05). The intragroup analysis revealed that HyFlex EDM had the highest cyclic fatigue resistance, followed by Reciproc Blue, WaveOne Gold, and Twisted File Adaptive in both air and water at 20°C (P<0.05). HyFlex EDM exhibited the highest cyclic fatigue resistance in water at 35°C, whereas there was no significant difference between the other groups (P>0.05). Conclusion. Within the limitations of the present study, an increase in the ambient temperature significantly decreased the cyclic fatigue resistance of the tested NiTi files.

Microcomputed Assessment of Transportation, Centering Ratio, Canal Area, and Volume Increase after Single-file Rotary and Reciprocating Glide Path Instrumentation in Curved Root Canals: A Laboratory Study.

INTRODUCTION: The aim of this study was to compare the transportation and centering abilities of the ProGlider (PG; Dentsply-Sirona, Ballaigues, Switzerland), WaveOne Gold Glider (WOGG; Dentsply-Sirona), and R-Pilot (RP; VDW, Munich, Germany) file systems and the changes in the structure model index (SMI), surface area, and root canal volume (after creating the glide path in the mandibular first molar teeth's mesial canals with moderate curvature) using the micro-computed tomographic (micro-CT) method. METHODS: In the present study, 24 mandibular first molar teeth with 2 separate mesial canals and moderate (10°-20°) curvature were used. After scanning with micro-CT imaging before the procedure, the teeth were divided into 3 groups, PG, WOGG, and RP (n = 10/each group), followed by the glide path procedure. After the root canal preparation, changes in the root canals regarding the volume, surface area, SMI, transportation made by the files, and the change of their centering ability in the apical, middle, and coronal third were analyzed by micro-CT imaging. The data were statistically evaluated using 1-way analysis of variance and the Kruskal-Wallis test with a significance level set at 5% (P < .05). RESULTS: The WOGG and RP file systems showed better centering abilities at all thirds compared with the PG file system (P < .05). No significant difference was found in the apical third between the groups in terms of canal transportation degree (P > .05). However, in the WOGG and RP groups, less transportation was observed in the middle and coronal thirds compared with the PG group (P < .05). When the SMI changed, after the root canal preparation was compared, no significant differences were found between the groups (P > .05). Nevertheless, the changes in volume and surface area of the root canal in the WOGG and RP groups were significantly lower than in the PG group (P < .05). CONCLUSIONS: The WOGG and RP file systems caused similar levels of change in the root canal systems based on all parameters. Moreover, it was determined that the PG file system caused a significantly higher volume and surface area increase than the WOGG and RP file systems.

Effect of Reciproc blue, XP-endo shaper, and WaveOne gold instruments on dentinal microcrack formation: A micro-computed tomographic evaluation.

The purpose of this study was to evaluate and compare the effect of Reciproc Blue (RPCB), XP-endo Shaper (XPS), and WaveOne Gold (WOG) single-files on dentinal microcrack formation using micro-computed tomography (micro-CT). Twenty-four mesial roots (24 mesio-buccal and 24 mesio-lingual canals) of mandibular molar teeth were prepared using RPCB, XPS, and WOG files (n = 8/each group). The samples were scanned with micro-CT in the pre- and post-preparation. Then, before preparation and after preparation cross-sectional images of the teeth were evaluated to detect the presence of microcracks. For each group, the number of microcracks was calculated as a percentage rate. The data were statistically analyzed using McNemar at 5% significance level (p < .05). Dentinal microcracks were observed in 25.99% (2,103 of 7,813), 31.99% (2,482 of 7,758), and 36.66% (2,836 of 7,731) of cross-sectional images of the XPS, WOG, and RPCB groups, respectively. In all the groups, all the dentinal microcracks seen in the post-treatment cross-sectional images were present in the corresponding pre-treatment images. Within the limitations of this study, the XPS, WOG, and RPCB files did not cause new dentinal microcrack formation or propagation of existing dentinal microcracks.

Influence of environmental temperature, heat-treatment and design on the cyclic fatigue resistance of three generations of a single-file nickel-titanium rotary instrument.

The aim of the present study was to evaluate the effect of environmental temperature, heat-treatment*** and design on the cyclic fatigue resistance of OneShape (OS), OneShape new generation (OSNG) and One Curve (OC) NiTi rotary single-file instruments. Cyclic fatigue testing of NiTi instruments was performed in an artificial canal with a 5-mm radius of curvature and a 60° angle of curvature located 5 mm from the tip of the instrument. During the experiment 3 different temperatures were used in a thermostatic bath with electronic and infrared controls: 0°, 20° and 35 °C. Sixty OneShape, 60 OneShape new generation and 60 One Curve instruments were rotated in the artificial canal until fracture occurred and the number of cycles to fracture (NCF) and the length of the fractured fragment (FL) was recorded. Data were analyzed by one-way ANOVA test at the 5% significance level. Fatigue resistance was significantly higher as the environmental temperature decreased, with differences ranging from 61 to 791%. OC instruments were significantly more resistant to cyclic fatigue than OS and OSNG instruments at all the temperatures tested (P < 0.05). There was no significant difference with respect to the length of the fractured file fragments for all the instruments tested at all the temperatures tested (P > 0.05). Within the limitation of the present study, fatigue resistance of the files was significantly increased when the environmental temperature decreased. OC instruments were significantly more resistant to cyclic fatigue than OS and OSNG instruments at all the temperatures tested.

Effects of etidronate, NaOCl, EDTA irrigation solutions and their combinations on cyclic fatigue resistance of nickel-titanium single-file rotary and reciprocating instruments at body temperature.

The aim of the present study was to compare the effect of etidronic acid (HEBP), NaOCl, and EDTA solutions and their combinations on cyclic fatigue resistance of NiTi RPC Blue (RPC Blue), HyFlex EDM (HEDM), and WaveOne Gold (WOG) instruments having different metallurgic characteristics at the body temperature (37 °C). 100 WOG, 100 RPC Blue, and 100 HEDM NiTi files were randomly divided into 5 groups and immersed in the following solutions: group 1, distilled water; group 2, 6% NaOCl; group 3, 17% EDTA; group 4, 18% HEBP; group 5, 6% NaOCl and 18% HEBP. Then the instruments were rotated in an artificial canal until fracture occurred and the number of cycles to fracture was calculated. A univariate analysis of variance and post hoc Tukey tests were then performed for comparisons using SPSS 21.0 software with the significance level set at P < .05. In all the conditions, there was no difference between the cyclic fatigue resistance of WOG and RPC Blue files (P > .05). In the intragroup comparison, WOG, RPC Blue, and HEDM files showed significantly low cyclic fatigue resistance in group 5 compared to other groups (P < .05). Among the groups, there was no statistically significant difference in the fracture lengths (P > .05). EDTA, NaOCl and HEBP solutions did not affect the cyclic fatigue resistance of the tested files when used without combination. However, the combination of NaOCl and HEBP solutions significantly reduced the cyclic fatigue resistance of the tested files.

Effect of chitosan nanoparticle, QMix, and EDTA on TotalFill BC sealers' dentinal tubule penetration: a confocal laser scanning microscopy study.

The aim of the present study was to compare the effect of chitosan nanoparticle, QMix, and 17% EDTA on the penetrability of a calcium silicate-based sealer into dentinal tubules using a confocal laser scanning microscope (CLSM). Sixty mandibular premolar teeth were selected and randomly divided into three groups (n = 20) before root canal preparation according to the solution used in the final rinse protocol: chitosan, QMix, and EDTA groups. Twenty teeth of each group were filled with a TotalFill BC sealers' single gutta-percha cone and with 0.1% rhodamine B. The specimens were horizontally sectioned at 3 and 5 mm from the apex, and the slices were analyzed in CLSM (4×). Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy with using Image J analysis software. Dentinal tubule's penetration depth, percentage, and area were measured using imaging software. Kruskal-Wallis test was used for statistical analysis. The level of significance was set at 5%. Results of Kruskal-Wallis analysis showed that there was a significant difference in the percentage and depth of sealer penetration among all groups at 3 and 5 mm level sections (P < 0.05). Within the groups, the minimum sealer penetration depth was recorded for chitosan nanoparticle group. Greater depth of sealer penetration was recorded at 5 mm as compared to 3 mm in all the groups. Within the limitation of the present study, it can be concluded that QMix and EDTA promoted sealer penetration superior to that achieved by chitosan nanoparticle.

Effect of Sodium Hypochlorite and EDTA on Surface Roughness of HyFlex CM and HyFlex EDM Files.

The aim of the present study was to compare the effect of exposure to sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA) on the surface topography and roughness of HyFlex controlled memory (CM) and HyFlex electrical discharge machining (EDM) using atomic force microscopy (AFM). Twenty HyFlex EDM (25/.08) and 20 HyFlex CM (25/.06) files were divided into five groups (n = 4). One group served as a negative control group. The files in the other groups were exposed to 5.25% NaOCl and 17% EDTA solutions at 37°C for 5 and 10 min, respectively, and examined using AFM after each exposure. The data were analyzed using one-way ANOVA test. The lowest values were observed in the control group of HyFlex EDM files (p < .05). The highest values were observed in the HyFlex EDM and HyFlex CM files immersed in EDTA for 10 min (p < .05). In conclusion, the tested irrigants had a significant negative effect on the surface porosity of NiTi files. The immersion of NiTi files in irrigants during cleaning and sterilization procedures results in corrosion and surface deformation of the files, and this can lead to unexpected file fracture during clinical use. Thus, the investigation of effect of ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) on surface topography of HyFlex files is important.

Cyclic Fatigue of Reciproc and Reciproc Blue Nickel-titanium Reciprocating Files at Different Environmental Temperatures.

INTRODUCTION: The aim of the present study was to investigate the impact of ambient temperature on the life span of nickel-titanium (NiTi) files. METHODS: Cyclic fatigue testing of NiTi instruments was performed in a stainless steel artificial canal. During the experiment, 4 different temperatures (0°C, 20°C, 35°C, and 39°C) were used in a thermostatic bath controlled using electronic and infrared controls. Eighty R25 Reciproc Blue (VDW, Munich, Germany) and 80 R25 Reciproc (VDW) instruments were reciprocated in the artificial canal until fracture occurred, and the time to fracture and the length of the fractured fragment were recorded. The phase transformation temperature for 3 instruments of each type was analyzed by differential scanning calorimetry. One-way analysis of variance and Bonferroni tests were used to statistically analyze the data at a 5% significance level (P < .05). RESULTS: Reciproc Blue instruments were significantly more resistant to cyclic fatigue than Reciproc at all the temperatures tested (P < .05). Fatigue resistance was proportionally higher as the environmental temperature decreased (0°C > 20°C > 35°C > 39°C). No significant difference was registered for the length of the fractured fragment of both instruments tested at all temperatures tested (P > .05). CONCLUSIONS: Blue alloy significantly increases the cyclic fatigue resistance of Reciproc files compared with M-Wire alloy. Temperature significantly affects the life span of NiTi files. When the ambient temperature increases between 0°C and 35°C, the fatigue resistance of the files statistically decreases.

Push-out bond strength of intra-orifice barrier materials: Bulk-fill composite versus calcium silicate cement.

Background. The aim of this study was to compare the push-out bond strengths of calcium silicate-based ProRoot MTA and Biodentine cements and SureFil SDR and EverX Posterior bulk-fill composite resins. Methods. Twenty-four single-rooted maxillary central incisors were sectioned below the cementoenamel junction, and the root canals were instrumented using rotary files. Thereafter, a parallel post drill was used to obtain a standardized root canal dimension. The roots were randomly assigned to one of the following groups with respect to the intra-orifice barrier used: ProRoot MTA; Biodentine; SureFil SDR; EverX Posterior. Five 1-mm-thick sections were obtained from the coronal aspect of each root. Push-out bond strength testing was performed and data were analyzed with Kruskal-Wallis and post hoc Dunn tests (P<0.05). Results. SureFil SDR and EverX Posterior bulk-fill composite resins' bond strengths were significantly higher than ProRoot MTA and Biodentine calcium silicate cements. However, no statistically significant differences were observed between bulk-fill composite resins values and calcium silicate cement values. Conclusion. Within the limitations of present study, calcium silicate-based ProRoot MTA cement's push-out bond strength was lower than those of Biodentine, SureFil SDR and EverX Posterior materials.